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yhdP [2018-07-04 10:23:31]
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yhdP [2018-07-04 10:23:31]

potential magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation
Locus
BSU09550
Isoelectric point
4.40
Molecular weight
49.69 kDa
Protein length
444 aa Sequence Blast
Gene length
1332 bp Sequence Blast
Function
export of magnesium
Product
potential magnesium efflux pump
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Magnesium uptake/ efflux
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,032,063 → 1,033,397

    The protein

    Protein family

  • UPF0053 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • YqhB, YrkA, YugS, YhdT

    • Domains

  • DUF21 domain (5-201)
  • 2 CBS domains (220-282, 284-344)

    • Structure

  • 4HG0 (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    yhdQ-mpfA
    Description
    PubMed

    Regulation

  • threefold induced in the presence of high Mg2+ concentrations PubMed
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    Biological materials

    Mutant

  • MGNA-A696 (yhdP::erm), available at the NBRP B. subtilis, Japan
  • BKE09550 (ΔmpfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
  • BKK09550 (ΔmpfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT

    • Expression vector

  • pGP2931 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

    • References

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