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yhdP [2019-06-26 09:46:15]
magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation
Molecular weight
49.69 kDa
Function
export of magnesium
Product
magnesium efflux pump
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,032,063 → 1,033,397
Phenotypes of a mutant
suppression of the growth defects of rplA and rplW mutants PubMedpartial suppression of sporulation frequency of a rplA mutant PubMed The protein
Protein family
UPF0053 family (according to Swiss-Prot)Paralogous protein(s)
Domains
Structure
4HG0 (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain) Localization
cell membrane (according to UniProt)Expression and Regulation
Biological materials
Mutant
MGNA-A696 (yhdP::erm), available at the NBRP B. subtilis, JapanBKE09550 (ΔmpfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGTBKK09550 (ΔmpfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT Expression vectors
pGP2931 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab References
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