SubtiBank SubtiBank
yhdP [2019-06-26 09:46:15]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

yhdP [2019-06-26 09:46:15]

magnesium efflux pump, mutation suppresses defects in 70 S ribosome formation
Locus
BSU09550
Isoelectric point
4.40
Molecular weight
49.69 kDa
Protein length
444 aa Sequence Blast
Gene length
1335 bp Sequence Blast
Function
export of magnesium
Product
magnesium efflux pump
Essential
no
Synonyms

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
1,032,063 → 1,033,397

Phenotypes of a mutant

  • suppression of the growth defects of rplA and rplW mutants PubMed
  • partial suppression of sporulation frequency of a rplA mutant PubMed
  • The protein

    Protein family

  • UPF0053 family (according to Swiss-Prot)
  • Paralogous protein(s)

    Domains

  • DUF21 domain (5-201)
  • 2 CBS domains (220-282, 284-344)
  • Structure

  • 4HG0 (CorC from E. coli, corresponds to the CBS domains and the C-terminal transporter-associated domain)
  • Localization

  • cell membrane (according to UniProt)
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulation

  • threefold induced in the presence of high Mg2+ concentrations PubMed
  • view in new tab

    Biological materials

    Mutant

  • MGNA-A696 (yhdP::erm), available at the NBRP B. subtilis, Japan
  • BKE09550 (ΔmpfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
  • BKK09550 (ΔmpfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATTAGAACCTTCACTCTA, downstream forward: _UP4_TAATAAGAAAAGCACCTTGT
  • Expression vectors

  • pGP2931 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
  • References

    Loading