steT [2018-09-13 18:40:16]

serine/ threonine exchanger transporter

locus: BSU12860

pI: 9.18

mw: 46.99 kDa

protein length: 438 aa

gene length: 1314 bp

function: exchange of serine and threonine

product: serine/ threonine exchanger transporter

essential: no

synonyms: ykbA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,351,375 → 1,352,691

The protein

Structure

• [PDB|6F34] (from Geobacillus kaustophilus, 25% identity) [pubmed|29416041]

[SW|Localization]

• membrane

Expression and Regulation

Operons

• genes: [gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]
  description: [Pubmed|22383849]

  regulation

  • expression activated by glucose (4.2 fold) [Pubmed|12850135]

Biological materials

Mutant

• MGNA-A736 (ykbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/736 NBRP B. subtilis, Japan]
• BKE12860 (Δ[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
• BKK12860 (Δ[gene|BCED6015E8CD17F94E9D0BC6CFEB15BF42F890A5|steT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

References

• 17344220,12850135,19419962,20610400,26976827,29416041