steT

serine/ threonine exchanger transporter

Locus: BSU_12860

Isoelectric point: 9.18

Molecular weight: 46.99 kDa

Protein length: 438 aa

Gene length: 1317 bp

Function: exchange of serine and threonine

Product: serine/ threonine exchanger transporter

Essential: no

Synonyms: ykbA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Amino acid transporters → APC superfamily
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,351,375 → 1,352,691

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Structure

• 6F34 (from Geobacillus kaustophilus, 25% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: steT
  Description: PubMed

  Regulation

  • expression activated by glucose (4.2 fold) PubMed

Biological materials

Mutant

• GP2378 ∆steT ::cat, available in Jörg Stülke's lab PubMed
• GP2945 ∆steT ::kan, available in Jörg Stülke's lab
• MGNA-A736 (ykbA::erm), available at the NBRP B. subtilis, Japan
• BKE12860 (ΔsteT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT
• BKK12860 (ΔsteT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCCTTAACCTCCTATG, downstream forward: _UP4_TGATAAAACGGTTCCCTTGT

Expression vector

• pGP2281: expression of steT by pBQ200 in B. subtilis, available in Jörg Stülke's lab

LacZ fusion

• pGP2275 (in pAC5) (GP2962), available in Jörg Stülke's lab

References

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