yvmC

cyclodipeptide synthase

Locus: BSU_35070

Isoelectric point: 6.61

Molecular weight: 28.36 kDa

Protein length: 248 aa

Gene length: 747 bp

Function: biosynthesis of the extracellular iron chelator pulcherrimin

Product: cyclodipeptide synthase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Acquisition of iron → Acquisition of iron / Other
• Metabolism → Additional metabolic pathways → Iron metabolism → Acquisition of iron / Other

Gene

Coordinates: 3,603,821 → 3,604,567

The protein

Catalyzed reaction/ biological activity

• synthesis of the cyclic dipeptide cyclo-L-leucyl-L-leucyl with the corresponding charged tRNAs as substrates in an ATP-dependent manner PubMed
• 2 L-leucyl-tRNA^Leu --> cyclo(L-leucyl-L-leucyl) + 2 H⁺ + 2 tRNA^Leu (according to UniProt)

Protein family

• CDPS family (single member, according to UniProt)

Structure

• 3S7T (from B. licheniformis, 70% identity, 86% similarity) PubMed

Expression and Regulation

Operons

• Genes: yvmC-cypX
  Description: Reference

  Regulatory mechanism

  • AbrB: repression, PubMed, in AbrB regulon
  • PchR: repression, in PchR regulon

  Regulation

  • expression of the operon is strongly induced at the beginning of biofilm formation PubMed

Biological materials

Mutant

• BKE35070 (ΔyvmC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCATTCACCCCTAAAA, downstream forward: _UP4_TGATAGGGGGAGTAAAACAT
• BKK35070 (ΔyvmC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTCATTCACCCCTAAAA, downstream forward: _UP4_TGATAGGGGGAGTAAAACAT

References

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