hutP

transcriptional antiterminator of the hut operon

Locus: BSU_39340

Isoelectric point: 6.06

Molecular weight: 16.43 kDa

Protein length: 151 aa

Gene length: 456 bp

Function: regulation of histidine utilization

Product: transcriptional antiterminator

Essential: no

Synonyms: hutP1

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of histidine
• Information processing → Regulation of gene expression → RNA binding regulators

Gene

Coordinates: 4,041,483 → 4,041,938

The protein

Protein family

• hutP family (single member, according to UniProt)

Cofactors

• Mg²⁺ PubMed

Effectors of protein activity

• HutP binds its RNA target to cause antitermination in the presence of histidine PubMed

Structure

• 3BOY (complex bound to the hut mRNA), 1WPT

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • CodY: repression, PubMed, in CodY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in HutP regulon

  Regulation

  • induced by histidine (HutP) PubMed

Biological materials

Mutant

• BKE39340 (ΔhutP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAATCACTCAATTCC, downstream forward: _UP4_TGAAACAGCCCATAGATCTT
• BKK39340 (ΔhutP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAATCACTCAATTCC, downstream forward: _UP4_TGAAACAGCCCATAGATCTT

References

Reviews

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Research papers

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