rpoC [2020-03-12 17:13:06]

RNA polymerase beta' subunit

Locus: BSU_01080

Isoelectric point: 8.86

Molecular weight: 133.92 kDa

Protein length: 1199 aa

Gene length: 3600 bp

Function: transcription

Product: RNA polymerase beta' subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → RNA polymerase
• Groups of genes → Essential genes
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 125,562 → 129,161

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate + RNA_(n) --> diphosphate + RNA_{(n + 1)} (according to UniProt)

Protein family

• RNA polymerase beta' chain family (single member, according to UniProt)

Modification

• phosphorylated on Arg-335 and Arg-803 PubMed

Structure

• 3IYD (RNA polymerase from E. coli, 50% identity) PubMed

Localization

• colocalizes with the nucleoid PubMed
• membrane associated PubMed

Expression and Regulation

Operons

• Genes: rpoB-rpoC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the leader mRNA is processed upstream of rpoB by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Expression vector

• pGP2180: expression of rpoC-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Reviews

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Original publications

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