rpoC [2017-05-09 15:36:36]

RNA polymerase beta subunit

locus: BSU01080

pI: 8.86

mw: 133.92 kDa

protein length: 1199 aa

gene length: 3597 bp

function: transcription

product: RNA polymerase beta subunit

essential: yes

ec: 2.7.7.6

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 125,562 → 129,161

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

Protein family

• [SW|RNA polymerase] beta' chain family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-335 and Arg-803 [Pubmed|22517742]

Structure

• [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 50% identity) [Pubmed|19903881]

[SW|Localization]

• colocalizes with the nucleoid [Pubmed|25092033]
• membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB]-[gene|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|rpoC]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7657605], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the leader mRNA is processed upstream of [gene|EC32328548BFB59D9026ACE351C946DBBC0932BC|rpoB] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Reviews

• 22210308,24763425

Original publications

• 7657605,12486038,10675340,7592585,19735077,18763711,19680289,22517742,23070162,20817769,24572018,25092033,24491578,26243774,21357417,26293966,26742846,19903881,17917675,26098117