rpoC [Sun Nov 27 2016 17:54:14 GMT+0100 (CET)]

RNA polymerase beta subunit

locus: BSU01080

pI: 8.00

mw: 133.00 kDa

function: transcription

product: RNA polymerase beta subunit

essential: yes

ec: 2.7.7.6

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

[SW|Categories] containing this gene/protein

• [SW|transcription], [SW|essential genes], [SW|membrane proteins], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|stringent response]

Gene

Coordinates on the chromosome (coding sequence): 125,562 -> 129,161

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate RNA(n) = diphosphate RNA(n 1) (according to Swiss-Prot)

Protein family

• [SW|RNA polymerase] beta' chain family (according to Swiss-Prot)

Modification

• phosphorylated on Arg-335 and Arg-803 [Pubmed|22517742]

Structure

• [PDB|3IYD] ([SW|RNA polymerase] from ''E. coli'', 50% identity) [Pubmed|19903881]

[SW|Localization]

• colocalizes with the nucleoid [Pubmed|25092033]
• membrane associated [Pubmed|18763711]

[SW|Interactions]

• [protein|search|RpoA]-[protein|search|RpoB]-[protein|search|RpoC] [Pubmed|10499798]
• [protein|search|RpoC]-[protein|search|RpoY] [Pubmed|25092033]
• [protein|search|SigA]-([protein|search|RpoB]-[protein|search|RpoC]) [Pubmed|19680289,19735077], [protein|search|SigB]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigD]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigE]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigF]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigG]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigH]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigI]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigK]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigL]-([protein|search|RpoB]-[protein|search|RpoC]-[protein|search|RpoA]) [Pubmed|26293966]
• [protein|search|SigM]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigV]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigW]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigX]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|SigY]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|SigZ]-([protein|search|RpoB]-[protein|search|RpoC])
• [protein|search|Xpf]-([protein|search|RpoB]-[protein|search|RpoC]), [protein|search|YlaC]-([protein|search|RpoB]-[protein|search|RpoC])
• [SW|YvrHa]-[protein|search|RpoC] [Pubmed|19940246]
• [protein|search|RpoC]-[protein|search|RpoE] (demonstrated for ''S. aureus'') [Pubmed|24491578]
• [SW|NusG]-[SW|RpoC] [Pubmed|26742846]
• [SW|GreA]-[SW|RpoC] [Pubmed|17917675]

Expression and Regulation

Operon

• ''[protein|search|rpoB]-[protein|search|rpoC]'' [Pubmed|22383849]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|7657605]

Regulation

• [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Reviews

• 22210308,24763425

Original publications

• 7657605,12486038,10675340,7592585,19735077,18763711,19680289,22517742,23070162,20817769,24572018,25092033,24491578,26243774,21357417,26293966,26742846,19903881,17917675