pupG

purine nucleoside phosphorylase

Locus: BSU_23490

Isoelectric point: 4.86

Molecular weight: 28.98 kDa

Protein length: 271 aa

Gene length: 816 bp

Function: purine salvage and interconversion

Product: purine nucleoside phosphorylase

Essential: no

E.C.: 2.4.2.1

Synonyms: punA,pnp,yqkO

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Purine salvage and interconversion
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 2,446,418 → 2,447,233

The protein

Catalyzed reaction/ biological activity

• purine 2'-deoxy-D-ribonucleoside + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + purine nucleobase (according to UniProt)

Protein family

• PNP/MTAP phosphorylase family (single member, according to UniProt)

Modification

• phosphorylation on Ser-28 PubMed

Structure

• 3LA8 (from Streptococcus mutans, 57% identity)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: drm-pupG
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

  Regulation

  • induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) PubMed

Biological materials

Mutant

• BKE23490 (ΔpupG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG
• BKK23490 (ΔpupG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TCTGTCCTTCAAGAAACAGT, downstream forward: _UP4_TAAATATGAATCAATGCAGG

References

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