yodC

NADH oxidase

locus: BSU_19550

pI: 5.27

mw: 22.19 kDa

protein length: 202 aa

gene length: 609 bp

function: regeneration of NAD from NADH

product: NADH oxidase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,127,813 → 2,128,421

The protein

Catalyzed reaction/ biological activity

• regeneration of NAD from NADH [Pubmed|26312069]

Protein family

• [SW|nitroreductase family] (according to UniProt)

Structure

• [PDB|3GE6] (from '' Exiguobacterium sibiricum'', 42% identity)

Expression and Regulation

Operons

• genes: [gene|BC434FE0E92376A618ED34ACBB07B8A2E7F31DE6|yodC]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB]: repression, [Pubmed|18208493], in [regulon|73AFDF65BE89C0E2CC59AF2AF64C29BD99D785DF|YodB regulon]

Biological materials

Mutant

• MGNA-B434 (yodC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1433 NBRP B. subtilis, Japan]
• BKE19550 (Δ[gene|BC434FE0E92376A618ED34ACBB07B8A2E7F31DE6|yodC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTGTTTCCTCCTTCGG, downstream forward: _UP4_TAAGGATATGAAAAACCTTA
• BKK19550 (Δ[gene|BC434FE0E92376A618ED34ACBB07B8A2E7F31DE6|yodC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTGTTTCCTCCTTCGG, downstream forward: _UP4_TAAGGATATGAAAAACCTTA

References

• 17407181,18208493,26312069