sdaAB

L-serine deaminase

locus: BSU_15850

pI: 5.10

mw: 23.67 kDa

protein length: 220 aa

gene length: 663 bp

function: serine utilization

product: L-serine deaminase (beta chain)

essential: no

ec: 4.3.1.17

synonyms: yloW,sdaA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]

Gene

Coordinates: 1,658,242 → 1,658,904

Phenotypes of a mutant

• overexpression of [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA] results in resistance to serine [pubmed|32743959]

The protein

Catalyzed reaction/ biological activity

• L-serine --> NH₄⁺ + pyruvate (according to UniProt)

Protein family

• iron-sulfur dependent L-serine dehydratase family (with [protein|8361D87307D2E8350945F592ABD24E2109E2E81A|SdaAA], according to UniProt)

[SW|Domains]

• [SW|ACT domain] (aa 148-220) (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Expression and Regulation

Operons

• genes: [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG
• BKK15850 (Δ[gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTATTCCTCCTTATGA, downstream forward: _UP4_TAGCGAAAAGGGTCAGGAGG

lacZ fusion

• pGP2275 (in [SW|pAC5]) (GP2967), available in [SW|Jörg Stülke]'s lab [pubmed|32743959]

References

• 22383849,22686449,24161940,32743959