glcT [Tue Jul 05 2016 18:02:36 GMT+0200 (CEST)]

transcriptional antiterminator , controls expression of the ptsG-ptsH-ptsI operon

locus: BSU13880

pI: 7.00

mw: 33.00 kDa

function: control of glucose uptake

product: transcriptional antiterminator of the ptsG-ptsH-ptsI operon

essential: no

synonyms: ykwA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

[SW|Categories] containing this gene/protein

• [SW|carbon core metabolism], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 1,456,092 -> 1,456,958

The protein

Catalyzed reaction/ biological activity

• [PRD-containing transcription factors|search|transcription antiterminator] , RNA-binding protein, binds the ''[protein|search|ptsG]'' RAT sequence

Protein family

• [PRD-containing transcription factors|search|transcription antiterminator] of the BglG/ [protein|search|SacY] family

Paralogous protein(s)

• [protein|search|LicT], [protein|search|SacT], [protein|search|SacY]

[SW|Domains]

• RNA-binding domain (N-terminal, constitutive antiterminator)
• 2x [SW|PTS] regulation domains (PRDs) (C-terminal, neg. regulated by [protein|search|PtsG])

Modification

• phosphorylation (His104)

Structure

• [PDB|3GWH] (PRD-II) [Pubmed|19684596]
• [PDB|3RIO] (RBD-PRD-I) [Pubmed|22750856]

[SW|Interactions]

• [protein|search|PtsH]-[protein|search|GlcT]

Biological materials

Mutant

• available in [SW|Jörg Stülke]'s lab:
• GP109 (in frame deletion)
• GP777 (replacement of ''[protein|search|glcT]'' and the ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' operon by a spc cassette), [Pubmed|22722928]
• GP926 (substitution of ''[protein|search|glcT]'' and ''[protein|search|ptsG]'' by a tet cassette), [Pubmed|22722928]

Expression vector

• pGP124 (full length, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP114 (amino acids 1-60, RNA-binding domain, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP164 (both PRDs, in [SW|pWH844]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP424 (PRDI, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP425 (PRDII, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP442 (PRDI, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
• pGP443 (PRDII, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
• pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1224 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1220 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Jörg Stülke], University of Göttingen, Germany
• [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

Reviews

• 9663674,18086213

Original publications

• 11902727,9765562,12437213,10543968,17074746,15155854,14527945,19684596,22722928,20939030,22750856