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glcT [2017-06-23 17:02:07]
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glcT [2017-06-23 17:02:07]

transcriptional antiterminator , controls expression of the ptsG-ptsH-ptsI operon
locus
BSU13880
pI
7.01
mw
33.03 kDa
protein length
285 aa Sequence Blast
gene length
855 bp Sequence Blast
function
control of glucose uptake
product
transcriptional antiterminator of the ptsG-ptsH-ptsI operon
essential
no
synonyms
ykwA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    1,456,092 → 1,456,958

    The protein

    Catalyzed reaction/ biological activity

  • [PRD-containing transcription factors|search|transcription antiterminator] , RNA-binding protein, binds the ''[gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]'' RAT sequence

    • Protein family

  • [PRD-containing transcription factors|search|transcription antiterminator] of the BglG/ [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY] family

    • Paralogous protein(s)

  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT], [protein|6796E1C147AA21E919A42A953884DC24E182F430|SacT], [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]

    • [SW|Domains]

  • RNA-binding domain (N-terminal, constitutive antiterminator)
  • 2x [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] regulation domains ([SW|PRD]s) (C-terminal, neg. regulated by [protein|B5E7EB475434E96786C577AE709A21BD702733D8|PtsG])

    • Modification

  • phosphorylation (His104)

    • Structure

  • [PDB|3GWH] ([SW|PRD]-II) [Pubmed|19684596]
  • [PDB|3RIO] (RBD-[SW|PRD]-I) [Pubmed|22750856]

    • Expression and Regulation

    Operons

    genes
    [gene|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|papB]-[gene|6FDE01EEFCDB2F949A902DFD76A341410B867E88|ykvZ]-[gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • available in [SW|Jörg Stülke]'s lab:
  • GP109 (in frame deletion)
  • GP777 (replacement of ''[gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]'' and the ''[gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]-[gene|29B793660E4D30C0656248F3EF403FEF76FB9025|ptsH]-[gene|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|ptsI]'' operon by a spc cassette), [Pubmed|22722928]
  • GP926 (substitution of ''[gene|BC0DF593AE969F1DBC5E9E2BACF1C00BE4FEE449|glcT]'' and ''[gene|B5E7EB475434E96786C577AE709A21BD702733D8|ptsG]'' by a tet cassette), [Pubmed|22722928]

    • Expression vector

  • pGP124 (full length, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP114 (amino acids 1-60, RNA-binding domain, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP230 (amino acids 1-60, RNA-binding domain with thrombin cleavage site, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP164 (both PRDs, in [SW|pWH844]), in addition diverse expression vectors for phosphorylation site mutants and for RBD mutants (all in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP424 (PRDI, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP425 (PRDII, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP442 (PRDI, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
  • pGP443 (PRDII, in [SW|pGP570], with thrombin cleavage site), available in [SW|Jörg Stülke]'s lab
  • pGP575 (amino acids 1-60, RNA-binding domain with Strep-tag, in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1224 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1220 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

    Reviews

  • 9663674,18086213

    • Original publications

  • 11902727,9765562,12437213,10543968,17074746,15155854,14527945,19684596,22722928,20939030,22750856