yrpC

glutamate racemase

locus: BSU_26810

pI: 6.74

mw: 29.73 kDa

protein length: 265 aa

gene length: 798 bp

function: peptidoglycan precursor biosynthesis

product: glutamate racemase

essential: no

ec: 5.1.1.3

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]

Gene

Coordinates: 2,738,308 → 2,739,105

The protein

Catalyzed reaction/ biological activity

• L-glutamate --> D-glutamate (according to UniProt)

Protein family

• aspartate/glutamate racemases family (with [protein|36FF9F097682D8BA8D7359BFEAF71ACE8E9E90D8|RacE] and [protein|2ABCC493C6CA847A7BC65B1200A9B6490621462A|RacX], according to UniProt)

Paralogous protein(s)

• [protein|36FF9F097682D8BA8D7359BFEAF71ACE8E9E90D8|RacE]

Structure

• [PDB|2JFN] (from ''Escherichia coli'', 36% identity, 54% similarity) [Pubmed|17568739]

Expression and Regulation

Operons

• genes: [gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]
  description: [Pubmed|22383849]

  regulation

  • expressed only in minimal medium, not in complex medium [Pubmed|15347750]

Biological materials

Mutant

• MGNA-A867 (yrpC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/867 NBRP B. subtilis, Japan]
• BKE26810 (Δ[gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCTCACGCTTTCT, downstream forward: _UP4_TGAATGTAAATGAAAAAGAC
• BKK26810 (Δ[gene|BBEE8CEC868D3B781BEA50C45935BC810EA7A4FB|yrpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTCTCACGCTTTCT, downstream forward: _UP4_TGAATGTAAATGAAAAAGAC

References

• 15347750,22383849,25777674