nrnB [2018-09-27 14:50:06]

oligoribonuclease (nano-RNase)

locus: BSU18200

pI: 5.03

mw: 46.28 kDa

protein length: 399 aa

gene length: 1197 bp

function: degradation of RNA oligonucleotides

product: nano-RNase B

essential: no

synonyms: yngD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 1,948,313 → 1,949,512

The protein

Catalyzed reaction/ biological activity

• degradation of RNA oligonucleotides [Pubmed|19553197]

Protein family

• DHH/DHHA1 protein family of phosphoesterases [Pubmed|19553197]

Expression and Regulation

Operons

• genes: [gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]
  description: [pubmed|22383849]

  regulation

  • expressed during [SW|sporulation] [pubmed|22383849]

Biological materials

Mutant

• MGNA-B398 (yngD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1397 NBRP B. subtilis, Japan]
• BKE18200 (Δ[gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG
• BKK18200 (Δ[gene|BBB0848B2516188E950763E1D0194860923CE895|nrnB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18200 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGTAGGCCCCTTTTT, downstream forward: _UP4_TAAAAAAATATGCATAGGGG

References

Reviews

• 29458847

Original publications

• 19553197