ytrI [2019-06-12 09:57:39]

spore cortex formation

locus: BSU29240

pI: 8.07

mw: 19.75 kDa

protein length: 176 aa

gene length: 531 bp

function: spore cortex formation

product: sporulation protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,995,067 → 2,995,597

Phenotypes of a mutant

• ''[gene|BBF22E368CB091678083934FD42BB11EA30B29DD|pdaB] [gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]'' double mutants are deficient in sporulation
• inactivation of ''[gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]'' reduces sporulation efficiency to 11.9% that of wild type cells [Pubmed|26735940]

The protein

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|D5B9ACB1E447A893C99A4ACE1DA95EB38BCF971B|ytrH]-[gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]
  description: [Pubmed|12662922]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]

Biological materials

Mutant

• MGNA-A430 (ytrI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/430 NBRP B. subtilis, Japan]
• BKE29240 (Δ[gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTTTTTTGTAATGCTGGGG, downstream forward: _UP4_TAATTATGGATTTTTTTTAA
• BKK29240 (Δ[gene|BB0443D95581DCA66EDA390842945F105A71A371|ytrI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTTTTTTGTAATGCTGGGG, downstream forward: _UP4_TAATTATGGATTTTTTTTAA

References

• 12662922,15547282,26735940