glnH [2018-05-04 13:24:03]

glutamine ABC transporter (binding protein)

Locus: BSU27440

Isoelectric point: 8.55

Molecular weight: 29.60 kDa

Protein length: 273 aa

Gene length: 819 bp

Function: glutamine uptake

Product: glutamine ABC transporter (binding protein)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of amino acids
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of glutamine/ glutamate
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,803,108 → 2,803,929

The protein

Structure

• 2V25 (from Campylobacter jejuni, 37% identity) PubMed

Localization

• attached to the membrane via GlnM-GlnP PubMed

Expression and Regulation

Operons

• Genes: glnQ-glnH-glnM-glnP
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • expressed during sporulation (SigE) PubMed

Biological materials

Mutant

• GP734 (spc), available in the Stülke lab
• BKE27440 (ΔglnH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA
• BKK27440 (ΔglnH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAAGCTCCCCCATTTC, downstream forward: _UP4_TAACAACCATCCCGGGATGA

References

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