rnc [2019-07-01 10:31:37]

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Locus: BSU15930

Isoelectric point: 8.08

Molecular weight: 28.28 kDa

Protein length: 249 aa

Gene length: 750 bp

Function: processing and degradation of RNA molecules

Product: endoribonuclease III

Essential: yes

Synonyms: rncS

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Endoribonucleases
• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation
• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Groups of genes → Essential genes

Gene

Coordinates: 1,665,710 → 1,666,459

Phenotypes of a mutant

• essential PubMed
• the rnc gene can be deleted in strains cured of the Skin element and SP-beta prophage (or upon deletion of the toxin genes txpA and yonT) PubMed

Additional information

• RNAs that are targeted by RNase III PubMed

The protein

Catalyzed reaction/ biological activity

• cleaves 16S rRNA 2 nts downstream of the 3' end PubMed
• cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
• degradation of txpA mRNA-FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0 RNA duplex (encoded on the Skin element) PubMed
• degradation of yonT mRNA-SR6 RNA duplex (encoded on the [[SP-beta prophage]]) PubMed
• degradation of the roxS-ytdI and roxS-sucC RNA duplexes PubMed

Structure

• 1O0W (from Thermotoga maritima, 41% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rnc-smc-ftsY
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Regulation

  • expressed during vegetative growth and during sporulation in the mother cell (SigK, GerE) PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab, PubMed

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
• Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original publications

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