rnc [Sat Jun 25 2016 16:04:17 GMT+0200 (CEST)]

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria

Locus: BSU15930

Isoelectric point: 8.00

Molecular weight: 28.00 kDa

Function: cleaves both 5- and 3-sites
of the small cytoplasmic RNA precursor (scr)

Product: endoribonuclease III

Essential: yes

Synonyms: rncS

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → RNases → Endoribonucleases
• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation
• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Groups of genes → Essential genes

Categories containing this gene/protein

• RNases, translation, protein secretion, essential genes

This gene is a member of the following regulons

• stringent response

Gene

Coordinates on the chromosome (coding sequence): 1,665,710 -> 1,666,459

Phenotypes of a mutant

• essential PubMed
• the rnc gene can be deleted in strains cured of the Skin element and [[SPß prophage]] (or upon deletion of the toxin genes txpA and yonT) PubMed

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
• cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
• degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
• degradation of yonT mRNA-as-yonT RNA duplex (encoded on the [[SP-beta prophage]]) PubMed
• degradation of the roxS-ppnKB and roxS-sucC RNA duplexes PubMed

Structure

• 1O0W (from Thermotoga maritima, 41% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• rnc-smc-ftsY PubMed

Sigma factor

• SigA PubMed

Regulation

• RelA dependent downregulation (Class I) during stringent response PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
• Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

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Original publications

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