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rnc [Sat Jun 25 2016 16:04:17 GMT+0200 (CEST)]
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rnc [Sat Jun 25 2016 16:04:17 GMT+0200 (CEST)]

RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
Locus
BSU15930
Isoelectric point
8.00
Molecular weight
28.00 kDa
Function
cleaves both 5- and 3-sites
of the small cytoplasmic RNA precursor (scr)
Product
endoribonuclease III
Essential
yes
Synonyms
rncS

Genomic Context

      
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Categories containing this gene/protein

Categories containing this gene/protein

This gene is a member of the following regulons

Gene

Coordinates on the chromosome (coding sequence)
1,665,710 -> 1,666,459

Phenotypes of a mutant

  • essential PubMed
  • the rnc gene can be deleted in strains cured of the Skin element and [[SPß prophage]] (or upon deletion of the toxin genes txpA and yonT) PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
  • cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
  • degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
  • degradation of yonT mRNA-as-yonT RNA duplex (encoded on the [[SP-beta prophage]]) PubMed
  • degradation of the roxS-ppnKB and roxS-sucC RNA duplexes PubMed
  • Structure

  • 1O0W (from Thermotoga maritima, 41% identity)
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operon

    Sigma factor

    Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • Biological materials

    Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Labs working on this gene/protein

    References

    Reviews

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    Original publications

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