yumC [2019-08-09 09:32:40]

ferredoxin/flavodoxin-NAD(P) oxidoreductase

locus: BSU_32110

pI: 5.57

mw: 36.68 kDa

protein length: 332 aa

gene length: 999 bp

function: redox reactions that involve ferredoxin and flavodoxin

product: ferredoxin/flavodoxin reductase

essential: yes

ec: 1.18.1.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,301,586 → 3,302,584

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2 reduced ferredoxin or flavodoxin NADP H = 2 oxidized ferredoxin or flavodoxin NADPH (according to Swiss-Prot)

Protein family

• ferredoxin--NADP reductase type 2 family (with [protein|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|YcgT], according to UniProt)

Paralogous protein(s)

• [protein|C35A302F0B0BC724EC91B47AB4D38CF56CADD574|YcgT]

Modification

• active site Cys85 is S-bacillithiolated by NaOCl stress in ''B. subtilis'' and other ''Bacillus'' species [http://www.ncbi.nlm.nih.gov/pubmed/22938038 PubMed]

[SW|Cofactors]

• FAD [Pubmed|20878669]

Structure

• [PDB|3LZW] [Pubmed|20878669]

Expression and Regulation

Operons

• genes: [gene|BAA74A6971CED9FB55540F1BC602CDA287D83E74|yumC]
  description: [Pubmed|22383849]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• MGNA-B572 (yumC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1571 NBRP B. subtilis, Japan]

References

• 22938038,20208166,20878669,19162251,15252706,24529496,25194416,25698107,26507228,26965753,26883633