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rapH [2018-10-15 12:48:06]
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rapH [2018-10-15 12:48:06]

response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay, control of ComA activity
locus
BSU06830
pI
6.12
mw
49.96 kDa
protein length
431 aa Sequence Blast
gene length
1293 bp Sequence Blast
function
control of sporulation initiation and ComA activity
product
response regulator aspartate phosphatase
essential
no
synonyms
yeeH, yzqA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.5|Phosphatases controlling the phosphorelay]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.5|Phosphatases controlling the phosphorelay]

    • Gene

    Coordinates
    750,959 → 752,089

    The protein

    Catalyzed reaction/ biological activity

  • binds to and thereby inhibits [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] activity [Pubmed|17581123]
  • RapH dephosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]-P and thereby controls the [SW|phosphorelay] [Pubmed|17581123]
  • in addition to dephosphorylating [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F], RapH can antagonize sporulation by sterically blocking phosphoryl transfer to and from [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F] [Pubmed|21346797]

    • Protein family

  • RAP family (according to Swiss-Prot)

    • Effectors of protein activity

  • interaction with [protein|4FA7D6E6316A6FC71C10C692D125833263894020|PhrH] inhibits dephosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F] and sequestration of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] by [protein|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|RapH] [Pubmed|21908671]

    • Structure

  • [PDB|3Q15] ([protein|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|RapH]-[protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F] complex) [Pubmed|21346797]

    • Expression and Regulation

    Operons

    genes
    [gene|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|rapH]-[gene|4FA7D6E6316A6FC71C10C692D125833263894020|phrH]
    description
    [Pubmed|21908671]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21908671], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR]: repression, [Pubmed|16553878], in [regulon|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR regulon]
  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • ''[protein|search|rapH]'': repressed by [protein|search|RghR] [Pubmed|16553878]
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    Biological materials

    Mutant

  • MGNA-A013 (rapH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/13 NBRP B. subtilis, Japan]
  • BKE06830 (Δ[gene|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|rapH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGCTTCCCTCCTTCTC, downstream forward: _UP4_GATATTCTAAAAGGAGAGTG
  • BKK06830 (Δ[gene|BA85E2C2E2A6B76FAFA31EBDBF466324C9B021C8|rapH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGGCTTCCCTCCTTCTC, downstream forward: _UP4_GATATTCTAAAAGGAGAGTG

    • References

  • 16553878,17581123,11948146,11918817,20817675,21346797,21908671