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queD
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queD

6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine
locus
BSU_13730
pI
5.94
mw
16.71 kDa
protein length
149 aa Sequence Blast
gene length
450 bp Sequence Blast
function
tRNA modification
product
6-pyruvoyltetrahydropterin synthase
essential
no
synonyms
ykvK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

    • Gene

    Coordinates
    1,440,100 → 1,440,549

    The protein

    Catalyzed reaction/ biological activity

  • 7,8-dihydroneopterin 3'-triphosphate + H2O --> 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H+ + triphosphate (according to UniProt)

    • Protein family

  • PTPS family (single member, according to UniProt)

    • Structure

  • [PDB|3QN0] (from E. coli, 33% identity) [pubmed|24816091]

    • Expression and Regulation

    Operons

    genes
    [gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
    description
    [Pubmed|14660578]

    regulatory mechanism

  • [regulon|preQ1 riboswitch|preQ1 riboswitch]: antitermination, in the absence of queuosine [Pubmed|19285444], in [regulon|preQ1 riboswitch|preQ1 riboswitch]

    • regulation

  • repressed in the presence of queuosine ([SW|preQ1 riboswitch]) [Pubmed|19285444]
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    Biological materials

    Mutant

  • MGNA-B325 (ykvK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1324 NBRP B. subtilis, Japan]
  • BKE13730 (Δ[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT, downstream forward: _UP4_TGAATGGCTAAAGGAATTCC
  • BKK13730 (Δ[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT, downstream forward: _UP4_TGAATGGCTAAAGGAATTCC

    • References

  • 16411777,18259064,14660578,19285444,17384645,24816091