SubtiBank SubtiBank
queD
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queD

6-pyruvoyltetrahydropterin synthase, synthesis of the modified ribonucleotide queuosine
Locus
BSU_13730
Isoelectric point
5.94
Molecular weight
16.71 kDa
Protein length
149 aa Sequence Blast
Gene length
450 bp Sequence Blast
Function
tRNA modification
Product
6-pyruvoyltetrahydropterin synthase
Essential
no
Synonyms
ykvK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation

    • Gene

    Coordinates
    1,440,100 → 1,440,549

    The protein

    Catalyzed reaction/ biological activity

  • 7,8-dihydroneopterin 3'-triphosphate + H2O --> 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H+ + triphosphate (according to UniProt)

    • Protein family

  • PTPS family (single member, according to UniProt)

    • Structure

  • 3QN0 (from E. coli, 33% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    queC-queD-queE-queF
    Description
    PubMed

    Regulatory mechanism

  • preQ1 riboswitch: antitermination, in the absence of queuosine PubMed, in preQ1 riboswitch

    • Regulation

  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed
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    Biological materials

    Mutant

  • MGNA-B325 (ykvK::erm), available at the NBRP B. subtilis, Japan
  • BKE13730 (ΔqueD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT, downstream forward: _UP4_TGAATGGCTAAAGGAATTCC
  • BKK13730 (ΔqueD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATTTATGCACGCTCTCCTTT, downstream forward: _UP4_TGAATGGCTAAAGGAATTCC

    • References

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