SubtiBank SubtiBank
ycbH
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ycbH

galactarate dehydratase
Locus
BSU_02510
Isoelectric point
5.70
Molecular weight
54.63 kDa
Protein length
510 aa Sequence Blast
Gene length
1533 bp Sequence Blast
Function
galactarate utilization
Product
galactarate dehydratase
Essential
no
E.C.
4.2.1.42
Synonyms
garD

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
274,029 → 275,561

The protein

Catalyzed reaction/ biological activity

  • galactarate --> 5-dehydro-4-deoxy-D-glucarate + H2O (according to UniProt)
  • Protein family

  • uxaA family (with UxaA, according to UniProt)
  • Structure

  • 6U7L (from E. coli, 67.6% identity)
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon
  • Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed
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    Biological materials

    Mutant

  • MGNA-C031 (ycbH::erm), available at the NBRP B. subtilis, Japan
  • BKE02510 (ΔycbH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG
  • BKK02510 (ΔycbH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG
  • References

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