SubtiBank SubtiBank
ycbH
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ycbH

galactarate dehydratase
Locus
BSU_02510
Isoelectric point
5.70
Molecular weight
54.63 kDa
Protein length
510 aa Sequence Blast
Gene length
1533 bp Sequence Blast
Function
galactarate utilization
Product
galactarate dehydratase
Essential
no
E.C.
4.2.1.42
Synonyms
garD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glucarate/galactarate

    • Gene

    Coordinates
    274,029 → 275,561

    The protein

    Catalyzed reaction/ biological activity

  • galactarate --> 5-dehydro-4-deoxy-D-glucarate + H2O (according to UniProt)

    • Protein family

  • uxaA family (with UxaA, according to UniProt)

    • Structure

  • 6U7L (from E. coli, 67.6% identity)

    • Expression and Regulation

    Operons

    Genes
    ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
    Description
    PubMed

    Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon

    • Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed
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    Biological materials

    Mutant

  • MGNA-C031 (ycbH::erm), available at the NBRP B. subtilis, Japan
  • BKE02510 (ΔycbH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG
  • BKK02510 (ΔycbH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGAAAATCACTCCTCA, downstream forward: _UP4_TGATTCATGTTTTTATAGCG

    • References

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