pstS

phosphate [SW|ABC transporter] (binding protein)

locus: BSU_24990

pI: 4.84

mw: 31.53 kDa

protein length: 300 aa

gene length: 903 bp

function: high-affinity phosphate uptake

product: phosphate [SW|ABC transporter] (binding protein)

essential: no

synonyms: yqgG,yzmB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

Gene

Coordinates: 2,580,715 → 2,581,617

The protein

Protein family

• pstS family (single member, according to UniProt)

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705]

Structure

• [PDB|4ECF] (from Lactobacillus brevis, 52% identity)

[SW|Localization]

• cell membrane (according to UniProt)
• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS]-[gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC]-[gene|5CE86C6768A4EF313FB5DBC270AE2A773EA58E81|pstA]-[gene|434BCED6953BE07D592AC229264C5AD0D04CBAF6|pstBA]-[gene|0D5BC94E21D51373A50D61CE3D0895B3DB28F6B3|pstBB]
  description: [Pubmed|9098050]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9098050], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]-P, [PubMed|9098050,9593301], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9098050,9593301 PubMed]

  additional information

  • mRNA processing between [gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS] and [gene|8BC7CCE9EBD899912ED7C72CA04FE82B21516F77|pstC] to maintain higher concentrations of [protein|BA45631351E3C9C140D15F620D7B7AC143C04932|PstS] relative to other components of the [SW|ABC transporter] [PubMed|15289558]

Biological materials

Mutant

• MGNA-C420 (yqgG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2418 NBRP B. subtilis, Japan]
• BKE24990 (Δ[gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTGAATTCCCCCTG, downstream forward: _UP4_TGATCTTAATAAAAAGAGGA
• BKK24990 (Δ[gene|BA45631351E3C9C140D15F620D7B7AC143C04932|pstS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTGAATTCCCCCTG, downstream forward: _UP4_TGATCTTAATAAAAAGAGGA

labs

• [SW|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

• 9098050,12897025,10913081,10092453,15289558,9098050,9593301,18957862,16493705,25666134