yngJ

3-methylbutanoyl-CoA dehydrogenase

Locus: BSU_18260

Isoelectric point: 5.79

Molecular weight: 40.76 kDa

Protein length: 380 aa

Gene length: 1143 bp

Function: mother cell metabolism, leucine utilization

Product: 3-methylbutanoyl-CoA dehydrogenase

Essential: no

E.C.: 1.3.99.10

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of branched-chain amino acids
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 1,956,218 → 1,957,360

The protein

Catalyzed reaction/ biological activity

• 3-methylbutanoyl-CoA -→ 3-methylbut-2-enoyl-CoA PubMed
• A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH₂ (according to UniProt)

Protein family

• Acyl-CoA dehydrogenase family (according to UniProt)

Paralogous protein(s)

• MmgC, AcdA, FadE

Cofactors

• FAD (according to UniProt)

Structure

• 3OWA (from B. anthracis, 37% identity, 54% similarity)

Expression and Regulation

Operons

• Genes: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

Biological materials

Mutant

• BKE18260 (ΔyngJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC, downstream forward: _UP4_TAAAAATGTAAACGCTTTCC
• BKK18260 (ΔyngJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATCGTTTCCTCCTTCAC, downstream forward: _UP4_TAAAAATGTAAACGCTTTCC

References

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