narG [2019-08-12 11:47:33]

nitrate reductase (alpha subunit)

locus: BSU_37280

pI: 6.06

mw: 138.86 kDa

protein length: 1228 aa

gene length: 3687 bp

function: nitrate respiration, nitrogen assimilation

product: nitrate reductase (alpha subunit)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.2|Anaerobic respiration]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,826,259 → 3,829,945

The protein

Catalyzed reaction/ biological activity

• Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)

Protein family

• [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [Pubmed|11289299]
• MoCo [Pubmed|11289299]

Structure

• [PDB|1Q16] (the [protein|B992698A12EE9D17DCEF075F82396D5456D35A20|NarG]-[protein|6181C785427277C097B81D67A93213AEAF701FF4|NarH]-[protein|20060F4C1939FB9A10CB279745D75D0A16627EC6|NarI] complex of E. coli, 52% identity) [pubmed|12910261]

[SW|Localization]

• cell membrane [Pubmed|23880299]

Expression and Regulation

Operons

• genes: [gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]-[gene|6181C785427277C097B81D67A93213AEAF701FF4|narH]-[gene|5528AD62C1BF608D181C8960C88620B63B1E60A0|narJ]-[gene|20060F4C1939FB9A10CB279745D75D0A16627EC6|narI]
  description: [Pubmed|8846791,16428414]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8846791,16428414], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr]: activation, [Pubmed|8846791,16428414], in [regulon|7165CC59CDAF64AAE2D591936860304A53BE5DF6|Fnr regulon]

  regulation

  • expressed under anaerobic conditions ([protein|search|Fnr]) [Pubmed|8846791,16428414]

Biological materials

Mutant

• BKE37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
• BKK37280 (Δ[gene|B992698A12EE9D17DCEF075F82396D5456D35A20|narG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA

References

Reviews

• 11289299

Original publications

• 9352926,23880299,8846791,16428414,12910261