SubtiBank SubtiBank
narG
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narG

nitrate reductase (alpha subunit)
Locus
BSU_37280
Isoelectric point
6.06
Molecular weight
138.86 kDa
Protein length
1228 aa Sequence Blast
Gene length
3687 bp Sequence Blast
Function
nitrate respiration, nitrogen assimilation
Product
nitrate reductase (alpha subunit)
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,826,259 → 3,829,945

The protein

Catalyzed reaction/ biological activity

  • quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
  • Protein family

    Domains

  • 4Fe-4S Mo/W bis-MGD-type domain (aa 47-111) (according to UniProt)
  • Cofactors

  • Fe-S cluster PubMed
  • MoCo PubMed
  • Structure

  • 1Q16 (the NarG-NarH-NarI complex of E. coli, 52% identity) PubMed
  • Localization

  • cell membrane PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Fnr: activation, PubMed, in Fnr regulon
  • Regulation

  • expressed under anaerobic conditions (Fnr) PubMed
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    Biological materials

    Mutant

  • BKE37280 (ΔnarG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
  • BKK37280 (ΔnarG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGAGAGTTCACTCCTTCC, downstream forward: _UP4_CGCAAGCTGAAGGAGGTCGA
  • References

    Reviews

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    Original publications

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