rlmCD

rRNA methyltransferase

Locus: BSU_06730

Isoelectric point: 5.71

Molecular weight: 51.65 kDa

Protein length: 459 aa

Gene length: 1380 bp

Function: 23S rRNA maturation

Product: rRNA methyltransferase

Essential: no

E.C.: 2.1.1.190

Synonyms: yerS,yefA

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation

Gene

Coordinates: 737,603 → 738,982

The protein

Catalyzed reaction/ biological activity

• methylation of U747 and U1939 in 23S rRNA PubMed
• S-adenosyl-L-methionine + uridine⁷⁴⁷ in 23S rRNA --> 5-methyluridine⁷⁴⁷ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)
• S-adenosyl-L-methionine + uridine¹⁹³⁹ in 23S rRNA --> 5-methyluridine¹⁹³⁹ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Paralogous protein(s)

• YfjO

Domains

• TRAM domain (aa 6-64) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 2BH2 (from E. coli, 32% identity) PubMed

Expression and Regulation

Operons

• Genes: dgkB-rlmCD
  Description: PubMed

• Genes: dgkB-rlmCD-yefC-dnmA-yeeB-yeeC
  Description: PubMed

Biological materials

Mutant

• MGNA-A931 (yefA::erm), available at the NBRP B. subtilis, Japan
• BKE06730 (ΔrlmCD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
• BKK06730 (ΔrlmCD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATAATCACCTCTAGT, downstream forward: _UP4_TTAAAAGAATAAATAGTCAA

References

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