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hemAT [2019-09-06 09:30:01]
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hemAT [2019-09-06 09:30:01]

soluble chemotaxis receptor, heme-containing O2 sensor protein
Locus
BSU_10380
Isoelectric point
5.44
Molecular weight
48.60 kDa
Protein length
432 aa Sequence Blast
Gene length
1299 bp Sequence Blast
Function
movement towards oxygen
Product
haem-based aerotactic transducer
Essential
no
Synonyms
yhfV
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble chemoreceptors
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for efficient pellicle biofilm formation

    • Gene

    Coordinates
    1,112,620 → 1,113,918

    Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
  • NCIB3610 hemAT mutant has higher fitness than hag mutant during pellicle formation PubMed
  • NCIB3610 hemAT-hag double mutant has similar fitness to single hag mutant during pellicle formation PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • required for full expression of the phoD-tatAD-tatCD operon PubMed

    • Cofactors

  • heme

    • Structure

  • 1OR4 (in cyano-liganded form), 1OR6 PubMed

    • Localization

  • forms clusters at the cell poles PubMed
  • homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase PubMed
  • polar foci in individual cells at later growth stages PubMed

    • Expression and Regulation

    Operons

    Genes
    hemAT
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

    • Additional information

  • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
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    Biological materials

    Mutant

  • MGNA-B282 (yhfV::erm), available at the NBRP B. subtilis, Japan
  • TB239 (hemAT::neo in 168) PubMed
  • TB241 (hemAT::neo in NCIB3610) PubMed
  • BKE10380 (ΔhemAT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATACAATGATCCCCCTTG, downstream forward: _UP4_TAACCATCAAAAACCGGTCT
  • BKK10380 (ΔhemAT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAATACAATGATCCCCCTTG, downstream forward: _UP4_TAACCATCAAAAACCGGTCT

    • Labs working on this gene/protein

  • Akos T Kovacs

    • References

    Reviews

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    Original publications

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