hemAT [2017-07-24 09:49:58]

soluble chemotaxis receptor, heme-containing O(2) sensor protein

locus: BSU10380

pI: 5.44

mw: 48.60 kDa

protein length: 432 aa

gene length: 1296 bp

function: movement towards oxygen

product: haem-based aerotactic transducer

essential: no

synonyms: yhfV

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.3|Soluble chemoreceptors]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation]

Gene

Coordinates: 1,112,620 → 1,113,918

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• NCIB3610 ''hemAT'' mutant has higher fitness than ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mutant during pellicle formation [Pubmed|26122431]
• NCIB3610 ''hemAT''-''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' double mutant has similar fitness to single ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' mutant during pellicle formation [Pubmed|26122431]

The protein

Catalyzed reaction/ biological activity

• required for full expression of the ''[gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]'' operon [Pubmed|23180473]

[SW|Cofactors]

• heme

Structure

• [PDB|1OR4] (in cyano-liganded form), [PDB|1OR6] [Pubmed|12962628]

[SW|Localization]

• forms clusters at the cell poles [Pubmed|21515776]
• homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase [Pubmed|23180473]
• polar foci in individual cells at later growth stages [Pubmed|23180473]

Expression and Regulation

Operons

• genes: [gene|B948B9D96CDC43A07B3FF90212E52230A0EF1905|hemAT]
  description: [Pubmed|15033535]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|15033535], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• MGNA-B282 (yhfV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1281 NBRP B. subtilis, Japan]
• TB239 (''hemAT''::''neo'' in 168) [Pubmed|26122431]
• TB241 (''hemAT''::''neo'' in NCIB3610) [Pubmed|26122431]

Labs working on this gene/protein

• [SW|Akos T Kovacs]

References

Reviews

• 23928310

Original publications

• 26122431,10676961,16819829,11481493,15033535,21515776,23180473,22564695