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hemAT [2017-8-11 16:0:3]
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hemAT [2017-8-11 16:0:3]

soluble chemotaxis receptor, heme-containing O2 sensor protein
Locus
BSU10380
Isoelectric point
5.44
Molecular weight
48.60 kDa
Protein length
432 aa Sequence Blast
Gene length
1296 bp Sequence Blast
Function
movement towards oxygen
Product
haem-based aerotactic transducer
Essential
no
Synonyms
yhfV
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Soluble chemoreceptors
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Other proteins required for efficient pellicle biofilm formation

    • Gene

    Coordinates
    1,112,620 → 1,113,918

    Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
  • NCIB3610 hemAT mutant has higher fitness than hag mutant during pellicle formation PubMed
  • NCIB3610 hemAT-hag double mutant has similar fitness to single hag mutant during pellicle formation PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • required for full expression of the phoD-tatAD-tatCD operon PubMed

    • Cofactors

  • heme

    • Structure

  • 1OR4 (in cyano-liganded form), 1OR6 PubMed

    • Localization

  • forms clusters at the cell poles PubMed
  • homogeneous cytoplasmic distribution in chain-forming cells during the logarithmic phase PubMed
  • polar foci in individual cells at later growth stages PubMed

    • Expression and Regulation

    Operons

    Genes
    hemAT
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

    • Additional information

  • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
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    Biological materials

    Mutant

  • MGNA-B282 (yhfV::erm), available at the NBRP B. subtilis, Japan
  • TB239 (hemAT::neo in 168) PubMed
  • TB241 (hemAT::neo in NCIB3610) PubMed

    • Labs working on this gene/protein

  • Akos T Kovacs

    • References

    Reviews

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    Original publications

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