gbsB [2019-08-09 08:28:09]

choline dehydrogenase (FAD-dependent), glycine betaine synthesis

locus: BSU_31050

pI: 4.91

mw: 43.39 kDa

protein length: 403 aa

gene length: 1209 bp

function: osmoprotection

product: choline dehydrogenase (FAD-dependent)

essential: no

ec: 1.1.1.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.4|Biosynthesis of glycine betaine]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

Gene

Coordinates: 3,183,867 → 3,185,075

The protein

Catalyzed reaction/ biological activity

• An alcohol + NAD⁺ = an aldehyde or ketone + NADH (according to Swiss-Prot)

Protein family

• iron-containing alcohol dehydrogenase family (with [protein|1E951C2B640966643D5F081159522CD1C51942C8|YugJ] and [protein|D81610C7CA2A92FCB1195591ACB613E7C1288F6B|YugK], according to UniProt)

[SW|Cofactors]

• FAD

Structure

• [PDB|3BFJ] (from Klebsiella pneumoniae, 39% identity) [pubmed|19011020]

Expression and Regulation

Operons

• genes: [gene|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA]-[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB]
  description: [Pubmed|8752328]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8752328], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]: repression, (transcriptional roadblock) [Pubmed|32849357,22408163], in [regulon|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR regulon]

  regulation

  • induced by choline ([protein|5C398C1181CE25CAC079AFBA5871C0B72AE1AA96|GbsR]) [Pubmed|22408163,8752328]

Biological materials

Mutant

• 1A1056 ( ''gbsB''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1056&Search=1A1056 BGSC]
• BKE31050 (Δ[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATGTCATCATCAATCTCC, downstream forward: _UP4_TAATCAAAATCCCGCTCCTT
• BKK31050 (Δ[gene|B90D4E798145F2AF3421DDC5AB6CD578E2E17900|gbsB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAATGTCATCATCAATCTCC, downstream forward: _UP4_TAATCAAAATCCCGCTCCTT

labs

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

• 8752328,21296969,22408163,29159878,19011020