rnhC [2017-07-24 14:09:22]

RNase HIII, endoribonuclease

locus: BSU28620

pI: 10.07

mw: 33.92 kDa

protein length: 313 aa

gene length: 939 bp

function: endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules

product: Mg2+-dependent RNase HIII

essential: no

synonyms: ysgB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

Gene

Coordinates: 2,926,031 → 2,926,972

Phenotypes of a mutant

• a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]'' double mutant grows poorly [Pubmed|23882084]

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)

Protein family

• RnhC subfamily (according to Swiss-Prot)

Structure

• [PDB|2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), [PDB|2D0A] (Geobacillus stearothermophilus, 47% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|B8B0F6B129475FDEEA8D538A51C153BF9A35887D|rnhC]
  description: [Pubmed|8969504]

Biological materials

Mutant

• MGNA-B002 (ysgB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1001 NBRP B. subtilis, Japan]
• BP430 (''ΔrnhC::cat'') available in [SW|Fabian Commichau]'s lab

Expression vector

• for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab

References

Reviews

• 19228197,24464998

Original publications

• 8969504,10094689,9888800,17905985,23882084,21710567