rnhC [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

RNase HIII, endoribonuclease

Locus: BSU28620

Isoelectric point: 10.07

Molecular weight: 33.00 kDa

Function: endonucleolytic cleavage of RNA
in RNA-DNA hybrid molecules

Product: Mg2+-dependent RNase HIII

Essential: no

Synonyms: ysgB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → RNA synthesis and degradation → RNases → Endoribonucleases

Categories containing this gene/protein

• RNAses

Gene

Coordinates on the chromosome (coding sequence): 2,926,031 -> 2,926,972

Phenotypes of a mutant

• a rnhB rnhC double mutant grows poorly PubMed

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)

Protein family

• RnhC subfamily (according to Swiss-Prot)

Structure

• 2D0B (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), 2D0A (Geobacillus stearothermophilus, 47% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Interactions

• RnhC interacts with the RNA polymerase PubMed

Expression and Regulation

Operon

• rnhC PubMed

Biological materials

Mutant

• BP430 (ΔrnhC::cat) available in Fabian Commichau's lab

Expression vector

• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab

References

Reviews

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Original publications

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