rnhC [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

RNase HIII, endoribonuclease

locus: BSU28620

pI: 10.07

mw: 33.00 kDa

function: endonucleolytic cleavage of RNA
in RNA-DNA hybrid molecules

product: Mg2+-dependent RNase HIII

essential: no

synonyms: ysgB

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

[SW|Categories] containing this gene/protein

• [SW|RNAses]

Gene

Coordinates on the chromosome (coding sequence): 2,926,031 -> 2,926,972

Phenotypes of a mutant

• a ''[protein|search|rnhB] [protein|search|rnhC]'' double mutant grows poorly [Pubmed|23882084]

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)

Protein family

• RnhC subfamily (according to Swiss-Prot)

Structure

• [PDB|2D0B] (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), [PDB|2D0A] (Geobacillus stearothermophilus, 47% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

[SW|Interactions]

• RnhC interacts with the [SW|RNA polymerase] [Pubmed|21710567]

Expression and Regulation

Operon

• ''[protein|search|rnhC]'' [Pubmed|8969504]

Biological materials

Mutant

• BP430 (''ΔrnhC::cat'') available in [SW|Fabian Commichau]'s lab

Expression vector

• for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in [SW|Fabian Commichau]'s lab

References

Reviews

• 19228197,24464998

Original publications

• 8969504,10094689,9888800,17905985,23882084,21710567