rnhC [2018-06-04 12:07:16]

RNase HIII, endoribonuclease

Locus: BSU28620

Isoelectric point: 10.07

Molecular weight: 33.92 kDa

Protein length: 313 aa

Gene length: 939 bp

Function: endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules, processing of R-loops

Product: Mn²⁺-dependent RNase HIII

Essential: no

E.C.: 3.1.26.4

Synonyms: ysgB

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Information processing → RNA synthesis and degradation → RNases → Endoribonucleases

Gene

Coordinates: 2,926,031 → 2,926,972

Phenotypes of a mutant

• snesitive to hydroxyurea PubMed
• a rnhB rnhC double mutant grows poorly PubMed

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of RNA to 5'-phosphomonoester (according to Swiss-Prot)

Protein family

• RnhC subfamily (according to Swiss-Prot)

Cofactors

• Mn²⁺ PubMed

Structure

• 2D0B (complex with Mg²⁺, Geobacillus stearothermophilus , 47% identity) PubMed
• 2D0A (Geobacillus stearothermophilus, 47% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• RnhC may interact with RNA polymerase PubMed

Expression and Regulation

Operons

• Genes: rnhC
  Description: PubMed

Biological materials

Mutant

• MGNA-B002 (ysgB::erm), available at the NBRP B. subtilis, Japan
• BP430 (ΔrnhC::cat) available in Fabian Commichau's lab
• BKE28620 (ΔrnhC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT
• BKK28620 (ΔrnhC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGTAATAATCTCCTTTTT, downstream forward: _UP4_TAGAAAAAAGCTTGCAGATT

Expression vector

• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from plasmid: pBP502 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab

References

Reviews

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Original publications

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