appF

oligopeptide ABC transporter (ATP-binding protein)

Locus: BSU_11370

Isoelectric point: 9.65

Molecular weight: 36.96 kDa

Protein length: 329 aa

Gene length: 990 bp

Function: uptake of oligopeptides

Product: oligopeptide ABC transporter (ATP-binding protein)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of peptides
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of nitrogen sources other than amino acids → Utilization of peptides
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,212,460 → 1,213,449

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• YkfD, OppF

Domains

• ABC transporter domain (aa 10-261) (according to UniProt)

Structure

• 4FWI (dipeptide transporter from Thermoanaerobacter tengcongensis, 37% identity) PubMed

Localization

• attached to the cell membrane (via AppB-AppC) PubMed

Expression and Regulation

Operons

• Genes: appD-appF-appA/1-appA/2-appB-appC
  Description: PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • ScoC: repression, PubMed, in ScoC regulon

  Regulation

  • repressed by CodY PubMed

Biological materials

Mutant

• GP2100 (D(appD-appF-appA/2-appB-appC)::spc), available in Jörg Stülke's lab
• BKE11370 (ΔappF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTCATGATTGTTCGGCACC, downstream forward: _UP4_TAATGTTGCCCGCACAGCTT
• BKK11370 (ΔappF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGTCATGATTGTTCGGCACC, downstream forward: _UP4_TAATGTTGCCCGCACAGCTT

References

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