appF

oligopeptide [SW|ABC transporter ](ATP-binding protein)

locus: BSU_11370

pI: 9.65

mw: 36.96 kDa

protein length: 329 aa

gene length: 990 bp

function: uptake of oligopeptides

product: oligopeptide [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.3|Uptake of peptides]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,212,460 → 1,213,449

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|YkfD], [protein|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|OppF]

[SW|Domains]

• [SW|ABC transporter domain] (aa 10-261) (according to UniProt)

Structure

• [PDB|4FWI] (dipeptide transporter from ''Thermoanaerobacter tengcongensis'', 37% identity) [Pubmed|23385461]

[SW|Localization]

• attached to the cell membrane (via [protein|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|AppB]-[protein|252E3F0A05D78CEC46A08B46D1C6133239B6B8BA|AppC]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|325BCDCAB4003204294229B7F9F0A5F353D72B54|appD]-[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]-[gene|DD2C5F549759447FF1C30E5B43BCB74CE4320EF3|appA/1]-[gene|082D2012ABE3671F62211F77ED501849BF77B4EF|appA/2]-[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]-[gene|252E3F0A05D78CEC46A08B46D1C6133239B6B8BA|appC]
  description: [Pubmed|7997159]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|10383984], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

  regulation

  • repressed by [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY] [Pubmed|12618455]

Biological materials

Mutant

• GP2100 (D(''[gene|325BCDCAB4003204294229B7F9F0A5F353D72B54|appD]-[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]-[gene|082D2012ABE3671F62211F77ED501849BF77B4EF|appA/2]-[gene|C6EB94EACDA544700A468545BD1FDA91C5FD82FB|appB]-[gene|252E3F0A05D78CEC46A08B46D1C6133239B6B8BA|appC]'')::''spc''), available in [SW|Jörg Stülke]'s lab
• BKE11370 (Δ[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATGATTGTTCGGCACC, downstream forward: _UP4_TAATGTTGCCCGCACAGCTT
• BKK11370 (Δ[gene|B88A88B4792FFFF4E1609B1FC91AAE6DBA339044|appF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTCATGATTGTTCGGCACC, downstream forward: _UP4_TAATGTTGCCCGCACAGCTT

References

• 12618455,7997159,10092453,10383984,12618455,25755103,23385461