splB [Mon Jan 02 2017 11:42:55 GMT+0100 (CET)]

spore photoproduct lyase

locus: BSU13930

pI: 8.00

mw: 39.00 kDa

function: protection of spore DNA against photodamage

product: spore photoproduct lyase

essential: no

synonyms: spl

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

[SW|Categories] containing this gene/protein

• [SW|DNA repair/ recombination], [SW|sporulation proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigG regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,461,770 -> 1,462,798

The protein

Catalyzed reaction/ biological activity

• repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase

[SW|Cofactors]

• contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM

Structure

• [PDB|4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) [Pubmed|22761404]

Expression and Regulation

Operon

• ''[protein|search|splA]-[protein|search|splB]'' [Pubmed|8021181]

[SW|Sigma factor]

• [protein|search|SigG] [Pubmed|15699190,8021181]

Regulation

• expressed during sporulation in the forespore ([protein|search|SigG]) [Pubmed|16497325]

Regulatory mechanism

• negative autoregulation by [protein|search|SplA] [Pubmed|8021181]

Biological materials

Mutant

• BP130 (del ''splB''::''spc''), available in [SW|Fabian Commichau]'s lab

References

Reviews

• 23164663,25477522

Structure of the spore photoproduct lesion in DNA

• 24598744

Original publications

• 15699190,8021181,21671623,22197590,23607538,23799365,22761404,28027411