pyrB [2019-08-01 09:54:33]

aspartate carbamoyltransferase

locus: BSU_15490

pI: 5.34

mw: 34.02 kDa

protein length: 304 aa

gene length: 915 bp

function: pyrimidine biosynthesis

product: aspartate carbamoyltransferase

essential: no

ec: 2.1.3.2

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 1,620,476 → 1,621,390

The protein

Catalyzed reaction/ biological activity

• Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate (according to Swiss-Prot)

Protein family

• ATCase/OTCase family (with [protein|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|ArgF], according to UniProt)

Modification

• phosphorylation on Ser-303 [Pubmed|17218307]

Structure

• [PDB|3R7D] [Pubmed|21663747]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]
  description: [Pubmed|8206849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1709162], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR]: termination/ antitermination, via [SW|RNA switch], in [regulon|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|PyrR regulon]

  regulation

  • induced in the absence of uridine nucleotides ([protein|search|PyrR]) [Pubmed|8206849]

Biological materials

Mutant

• BKE15490 (Δ[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCCCTCTCTT, downstream forward: _UP4_AATGTGAAAAGAGGAGAAGC
• BKK15490 (Δ[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCCCTCTCTT, downstream forward: _UP4_AATGTGAAAAGAGGAGAAGC

References

• 7868607,8206849,12896995,3015959,7840626,6427186,1709162,3937019,17218307,17322189,21663747