SubtiBank SubtiBank
yxjF [2018-02-13T12:18:45.000Z]
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yxjF [2018-02-13T12:18:45.000Z]

sporulation protein, 3-hydroxybutyrate dehydrogenase
locus
BSU38970
pI
7.89
mw
27.46 kDa
protein length
257 aa Sequence Blast
gene length
771 bp Sequence Blast
function
3-hydroxybutyrate utilization
product
3-hydroxybutyrate dehydrogenase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    4,000,539 → 4,001,312

    The protein

    Protein family

  • short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|D945CAD7209CB94A7B9E23263476124B7E8853DD|YusZ], [protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|YxbG] (35%)

    • Structure

  • [PDB|2YZ7] (from Alcaligenes Faecalis 42% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|B416BB368A96926526458B2258BF8AB928D7C15E|hbuT]-[gene|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]-[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]
    description
    [Pubmed|10746760]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135,10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|12662922]
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    Biological materials

    Mutant

  • MGNA-B730 (yxjF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1729 NBRP B. subtilis, Japan]
  • GP1112 (spc), available in [SW|Stülke] lab
  • BKE38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG
  • BKK38970 (Δ[gene|B7B1D28E50244704324920E5A6F110DA8D63F6C0|yxjF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG

    • References

  • 16672620,10666464,12850135,10746760,12662922