SubtiBank SubtiBank
yxjF
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yxjF

sporulation protein, 3-hydroxybutyrate dehydrogenase
Locus
BSU_38970
Isoelectric point
7.89
Molecular weight
27.46 kDa
Protein length
257 aa Sequence Blast
Gene length
774 bp Sequence Blast
Function
3-hydroxybutyrate utilization
Product
3-hydroxybutyrate dehydrogenase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    4,000,539 → 4,001,312

    The protein

    Protein family

  • Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

    • Paralogous protein(s)

  • YusZ
  • YxbG:

    • Structure

  • 2YZ7 (from Alcaligenes Faecalis 42% identity)

    • Expression and Regulation

    Operons

    Genes
    hbuT-scoA-scoB-yxjF
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon

    • Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed
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    Biological materials

    Mutant

  • MGNA-B730 (yxjF::erm), available at the NBRP B. subtilis, Japan
  • GP1112 (spc), available in Stülke lab
  • BKE38970 (ΔyxjF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG
  • BKK38970 (ΔyxjF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCAAACACCTTCCCAT, downstream forward: _UP4_TGACATAAAAAACACACTTG

    • References

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