catD

essential for viability in the presence of catechol

Locus: BSU_08230

Isoelectric point: 10.11

Molecular weight: 14.28 kDa

Protein length: 134 aa

Gene length: 405 bp

Function: detoxification of catechol

Product: unknown

Essential: no

Synonyms: yfiD

Genomic Context

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Categories containing this gene/protein

• Metabolism → Detoxification reactions
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Membrane proteins

Gene

Coordinates: 897,588 → 897,992

Phenotypes of a mutant

• not viable in the presence of catechol PubMed

The protein

Protein family

• DoxX family (with MhqP, according to UniProt)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: catD-catE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • YodB: repression, PubMed, in YodB regulon
  • CatR: repression, PubMed, in CatR regulon
  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • strong induction by diamide and quinones (such as catechol) (YodB, CatR) PubMed
  • induced by iron starvation (Fur) PubMed

Biological materials

Mutant

• MGNA-C294 (yfiD::erm), available at the NBRP B. subtilis, Japan
• BKE08230 (ΔcatD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTATCCTCCTTGATAA, downstream forward: _UP4_GCATAAGAAAAGAGGCATGC
• BKK08230 (ΔcatD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTATCCTCCTTGATAA, downstream forward: _UP4_GCATAAGAAAAGAGGCATGC

References

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