yfkN [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

cell wall-anchored 3'5' cyclic dinucleotide 3' phosphodiesterase, degrades c-di-AMP

locus: BSU07840

pI: 5.00

mw: 159.00 kDa

function: degradation of c-di-AMP

product: cell wall-anchored 3'5' cyclic dinucleotide 3′ phosphodiesterase, degrades c-di-AMP

essential: yes

ec: 3.1.4.16

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

[SW|Categories] containing this gene/protein

• [SW|metabolism of signalling nucleotides], [SW|essential genes], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|PhoP regulon]

Gene

Coordinates on the chromosome (coding sequence): 855,114 -> 859,502

Phenotypes of a mutant

• essential [Pubmed|17114254]

The protein

Catalyzed reaction/ biological activity

• degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]

Paralogous protein(s)

• [protein|search|YhcR]

[SW|Cofactors]

• Mn2 [Pubmed|27414497]

Structure

• [PDB|3GVE]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862], the transmembrane domain serves as retention signal, attached to the cell wall [Pubmed|21800427,22767609]

[SW|Interactions]

• [protein|search|YhcS]-[protein|search|YfkN] [Pubmed|21800427] (anchoring to the cell wall)

Expression and Regulation

Operon

• ''yfkN'' [Pubmed|16291680]

Regulation

• repressed by glucose (5.2-fold) ([protein|search|CcpA]) [Pubmed|12850135]
• expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]

Regulatory mechanism

• [protein|search|CcpA]: transcription repression [Pubmed|12850135]
• [protein|search|PhoP]: transcription activation [Pubmed|16291680]

References

• 14688230,16291680,18957862,12850135,17114254,21800427,27414497