cell wall-anchored 3'5' cyclic dinucleotide 3' phosphodiesterase, degrades c-di-AMP
function
degradation of c-di-AMP
product
cell wall-anchored 3'5' cyclic dinucleotide 3′ phosphodiesterase, degrades c-di-AMP
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|metabolism of signalling nucleotides], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|PhoP regulon]Gene
Coordinates on the chromosome (coding sequence)
855,114 -> 859,502
Phenotypes of a mutant
essential according to Kobayshi ''et al.'' [Pubmed|17114254], but a deletion mutant could be constructed [Pubmed|27681641]The protein
Catalyzed reaction/ biological activity
degrades c-di-AMP two 5′ AMP molecules [Pubmed|27414497]Paralogous protein(s)
[protein|search|YhcR][SW|Cofactors]
Structure
[PDB|3GVE][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862], the transmembrane domain serves as retention signal, attached to the cell wall [Pubmed|21800427,22767609][SW|Interactions]
[protein|search|YhcS]-[protein|search|YfkN] [Pubmed|21800427] (anchoring to the cell wall)Expression and Regulation
Operon
''yfkN'' [Pubmed|16291680]Regulation
repressed by glucose (5.2-fold) ([protein|search|CcpA]) [Pubmed|12850135]expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]Regulatory mechanism
[protein|search|CcpA]: transcription repression [Pubmed|12850135][protein|search|PhoP]: transcription activation [Pubmed|16291680]Biological materials
Mutant
MGNA-C263 (yfkN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2261 NBRP B. subtilis, Japan]References
14688230,16291680,18957862,12850135,17114254,21800427,27414497,27681641 
