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rnjB [2018-04-15 14:00:56]
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rnjB [2018-04-15 14:00:56]

RNase J2
Locus
BSU16780
Isoelectric point
9.18
Molecular weight
56.67 kDa
Protein length
515 aa Sequence Blast
Gene length
1545 bp Sequence Blast
Function
RNA processing and degradation
Product
RNase J2
Essential
no
Synonyms
ymfA
Outlinks
KEGGBsubCycSubtiListExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → RNases → Endoribonucleases

    • Gene

    Coordinates
    1,749,418 → 1,751,085

    Phenotype of a mutant

  • growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • endoribonuclease, involved in processing of thrS mRNA

    • Protein family

  • RNase J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • RnjA

    • Structure

  • 3ZQ4, RNase J1, 49% identity, 81% similarity PubMed

    • Localization

  • cytoplasm, colocalizes with the ribosomes PubMed

    • Expression and Regulation

    Operons

    Genes
    rnjB
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulation

  • constitutive expression PubMed
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    Biological materials

    Mutant

  • MGNA-B114 (ymfA::erm), available at the NBRP B. subtilis, Japan
  • GP1113 (rnjB::miniTn10 spc), available in Jörg Stülke's lab
  • GP1291 (ΔrnjB::cat), available in Jörg Stülke's lab
  • BKE16780 (ΔrnjB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAATCTATATCCTCCTAG, downstream forward: _UP4_TAATGACTGACTAAAGACCG
  • BKK16780 (ΔrnjB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAATCTATATCCTCCTAG, downstream forward: _UP4_TAATGACTGACTAAAGACCG

    • Expression vectors

  • GP1687, chromosomal expression of rnjB-Strep::kan in B. subtilis, available in Jörg Stülke's lab
  • GP1724, chromosomal expression of rnjB-Strep::spc in B. subtilis, based on pGP1389, available in Jörg Stülke's lab
  • pGP1438: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
  • pGP1440: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

    • LacZ fusion

  • pGP419 (in pAC7), available in Jörg Stülke's lab

    • GFP fusion

  • GP1695 (in pHJS-105 PubMed), expression of rnjB-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
  • GP1699 (in pBP43), expression of rnjB-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

    • FLAG-tag construct

  • GP1001 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Harald Putzer, IBPC Paris, France Homepage

    • References

    Reviews

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    Original publications

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