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rnjB [Fri Feb 03 2017 13:26:52 GMT+0100 (CET)]
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rnjB [Fri Feb 03 2017 13:26:52 GMT+0100 (CET)]

RNase J2
locus
BSU16780
pI
9.00
mw
56.00 kDa
function
RNA processing and degradation
product
RNase J2
essential
no
synonyms
ymfA
Outlinks
KEGGBsubCycSubtiListExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]

    • [SW|Categories] containing this gene/protein

  • [SW|RNAses]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,749,418 -> 1,751,085

    Phenotype of a mutant

  • growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) [Pubmed|26296725]

    • The protein

    Catalyzed reaction/ biological activity

  • endoribonuclease, involved in processing of ''[protein|search|thrS]'' mRNA

    • Protein family

  • [SW|RNase] J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|RnjA]

    • Structure

  • [PDB|3ZQ4], RNase J1, 49% identity, 81% similarity [Pubmed|21893285]

    • [SW|Localization]

  • cytoplasm, colocalizes with the ribosomes [Pubmed|27708634]

    • [SW|Interactions]

  • associated to the [SW|RNA degradosome] [Pubmed|19193632]
  • [protein|search|RnjA]-[protein|search|RnjB] [Pubmed|19193632,20025672,27708634]

    • Expression and Regulation

    Operon

  • ''[protein|search|rnjB]'' [Pubmed|24187087]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|24187087]

    • Regulation

  • constitutive expression [Pubmed|24187087]

    • Biological materials

    Mutant

  • MGNA-B114 (ymfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1113 NBRP B. subtilis, Japan]
  • GP1113 (miniTn10 spc), available in [SW|Jörg Stülke]'s lab
  • GP1291 (rnjB::cat), available in [SW|Jörg Stülke]'s lab

    • Expression

  • GP1687, expression of '' rnjB''-Strep::''kan'' in '' B. subtilis'', available in [SW|Jörg Stülke]'s lab
  • GP1724, expression of '' rnjB''-Strep::''spc '' in '' B. subtilis'', based on [SW|pGP1389], available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP419 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1695 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjB-sfGFP''::''spc'' under xylose-inducible promoter in '' B. subtilis'', available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1001 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]

    • References

    Reviews

  • 20458164,21893280,23403287,21957024,22568516

    • Original publications

  • 15831787,18204464,18713320,19193632,19633085,20025672,21862575,21893285,24187087,26296725,27708634