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rnjB [Fri Feb 03 2017 13:25:28 GMT+0100 (CET)]
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rnjB [Fri Feb 03 2017 13:25:28 GMT+0100 (CET)]

RNase J2
Locus
BSU16780
Isoelectric point
9.00
Molecular weight
56.00 kDa
Function
RNA processing and degradation
Product
RNase J2
Essential
no
Synonyms
ymfA
Outlinks
KEGGBsubCycSubtiListExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → RNases → Endoribonucleases

    • Categories containing this gene/protein

  • RNAses

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,749,418 -> 1,751,085

    Phenotype of a mutant

  • growth advantage at low pressure and at 27°C at normal pressure (has been selected at low pressure) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • endoribonuclease, involved in processing of thrS mRNA

    • Protein family

  • RNase J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • RnjA

    • Structure

  • 3ZQ4, RNase J1, 49% identity, 81% similarity PubMed

    • Localization

  • cytoplasm, colocalizes with the ribosomes PubMed

    • Interactions

  • associated to the RNA degradosome PubMed
  • RnjA-RnjB PubMed

    • Expression and Regulation

    Operon

  • rnjB PubMed

    • Sigma factor

  • SigA PubMed

    • Regulation

  • constitutive expression PubMed

    • Biological materials

    Mutant

  • MGNA-B114 (ymfA::erm), available at the NBRP B. subtilis, Japan
  • GP1113 (miniTn10 spc), available in Jörg Stülke's lab
  • GP1291 (rnjB::cat), available in Jörg Stülke's lab

    • Expression

  • GP1687, expression of rnjB-Strep::kan in B. subtilis, available in Jörg Stülke's lab
  • GP1724, expression of rnjB-Strep::spc in B. subtilis, based on pGP1389, available in Jörg Stülke's lab

    • LacZ fusion

  • pGP419 (in pAC7), available in Jörg Stülke's lab

    • GFP fusion

  • GP1695 (in pHJS-105 PubMed), expression of rnjB-sfGFP::kan under xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP1001 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Harald Putzer, IBPC Paris, France Homepage

    • References

    Reviews

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    Original publications

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