ytdI

inorganic polyphosphate/ATP-NAD kinase

Locus: BSU_29540

Isoelectric point: 6.49

Molecular weight: 30.10 kDa

Protein length: 267 aa

Gene length: 804 bp

Function: generation of NADP from NAD

Product: inorganic polyphosphate/ATP-NAD kinase

Essential: no

E.C.: 2.7.1.23

Synonyms: ppnKB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of NAD(P)

Gene

Coordinates: 3,021,233 → 3,022,036

The protein

Catalyzed reaction/ biological activity

• ATP + NAD⁺ --> ADP + H⁺ + NADP⁺ (according to UniProt)

Protein family

• NAD kinase family (with NadF, according to UniProt)

Paralogous protein(s)

• NadF

Structure

• 1YT5 (from Thermotoga maritima) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ytdI
  Description: PubMed

  Additional information

  • half-life of the mRNA: 8.4 min, in a roxS mutant 15 min, in a rny mutant 24 min PubMed

Other regulations

• RoxS: translation inhibition, inhibition of translation and initiation of RNA degradation by Rny and Rnc PubMed translation is inhibited by binding of the RoxS RNA to the mRNA PubMed

Biological materials

Mutant

• MGNA-A109 (ytdI::erm), available at the NBRP B. subtilis, Japan
• BKE29540 (ΔytdI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCCATTTCCCCTTTG, downstream forward: _UP4_TAGAAAATAAAAGGACAGGC
• BKK29540 (ΔytdI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCCCATTTCCCCTTTG, downstream forward: _UP4_TAGAAAATAAAAGGACAGGC

References

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