SubtiBank SubtiBank
ligB
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ligB

DNA ligase (ATP-dependent)
locus
BSU_20500
pI
6.86
mw
30.88 kDa
protein length
270 aa Sequence Blast
gene length
813 bp Sequence Blast
function
phage SP-beta replication
product
DNA ligase (ATP-dependent)
essential
no
synonyms
yoqV

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]
  • Gene

    Coordinates
    2,192,370 → 2,193,182

    The protein

    Protein family

  • ATP-dependent DNA ligase family (C-terminal domain) (with [protein|127CB0930A2D96B18D9261180028818F158C68CB|LigD], according to UniProt)
  • Structure

  • [PDB|6BKF] (human protein, 24% identity) [pubmed|29980672]
  • Biological materials

    Mutant

  • BKE20500 (Δ[gene|B66132676788FBA47D375193A1C01FC6F93E110F|ligB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE20500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATATCACCTCCTACTT, downstream forward: _UP4_TAATCTCTCCGTTACATAAC
  • BKK20500 (Δ[gene|B66132676788FBA47D375193A1C01FC6F93E110F|ligB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK20500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAATATCACCTCCTACTT, downstream forward: _UP4_TAATCTCTCCGTTACATAAC
  • References

  • 11095673,29980672