pucD [2017-8-11 16:0:3]

xanthine dehydrogenase

locus: BSU32480

pI: 5.75

mw: 80.25 kDa

protein length: 745 aa

gene length: 2235 bp

function: purine utilization

product: xanthine dehydrogenase

essential: no

synonyms: yurC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

Gene

Coordinates: 3,336,263 → 3,338,500

The protein

Catalyzed reaction/ biological activity

• Xanthine + NAD⁺ + H₂O = urate + NADH (according to Swiss-Prot)

Protein family

• xanthine dehydrogenase family (according to Swiss-Prot)

Structure

• [PDB|1RM6] (4-hydroxybenzoyl-CoA reductase from Thauera aromatica, 30% identity) [pubmed|15576037]

Expression and Regulation

Operons

• genes: [gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
  description: [Pubmed|12823818]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

  regulation

  • induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]

Biological materials

Mutant

• MGNA-A964 (yurC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/964 NBRP B. subtilis, Japan]

References

• 11344136,12029039,12823818,15576037